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. 1998 Oct 5;143(1):207–215. doi: 10.1083/jcb.143.1.207

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Failure of BAX to target mitochondria in vitro depends on the BAX TM. cDNAs encoding BAX (lanes 2–5), cytosolic dihydrofolate reductase fused at its COOH terminus to amino acids 169– 192 of BAX (DHFR-BAX™, lanes 7–10), and BAX in which the COOH-terminal 24 amino acids have been replaced by the corresponding domain of BCL-2 (amino acids 218–239) (BAX-BCL-2™, lanes 12–15) were transcribed and translated in reticulocyte lysate and the ³⁵S-labeled, alkali-insoluble mitochondrial import products were analyzed by SDS PAGE and fluorography. Lanes 1, 6, and 11 represent 10% of input translation product, as indicated. The sequence (single letter code) of the COOH-terminal 24 amino acids of BAX and 22 amino acids of BCL-2 are shown; bold letters designate the predicted TMs.