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. 1998 Oct 5;143(1):13–22. doi: 10.1083/jcb.143.1.13

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Concurrent FISH and fluorescent BrdU detection to measure chromosome position. (A) Diagram of chromosome 2 from bw^D and wild type with the locations of the genomic probes indicated. Black region is heterochromatic. The circle represents the centromere. (B) Two panels of interphase nuclei from the CNS of a bw^D larva. DAPI staining is in blue. (Top) Hybridization of the two probes in A. (Bottom) The same nuclei with the signal from anti-BrdU fluorescein indicating those nuclei that have incorporated DNA within the last 4–5 h. Differential and weak DAPI staining (relative to anti-BrdU staining) are responsible for the seeming discordance of DNA localization, FISH signal and BrdU incorporation.