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. 1998 Oct 5;143(1):135–145. doi: 10.1083/jcb.143.1.135

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Spindle positioning in cdc28-4 clb5Δ mutants. (A) Zygotes from parental or mutant crosses. Mating assays with cells expressing GFP:TUB1 were performed as described for Fig. 2 except that cells were visualized without fixation. Pairs of DIC and GFP fluorescence images are shown of representative stages for cdc28-4 cells: G2 (a), early anaphase (b), late anaphase (c), and cdc28-4 clb5 short and intermediate length spindles positioned in the bud (d–i). Bar, 5 μm. (B) Diploid cdc28-4 GAL1:CLB5 (MYT1010; a–c) or cdc28-4 clb5 GAL1:CLB5 (MYT1416; d–f) expressing GFP:TUB1 after a 6-h shift from galactose to dextrose containing medium at 23°C. Representative stages are shown for parental SPB duplication and G2 spindle (a), G2 and midanaphase spindles (b), late anaphase and spindle disassembly (c), and mutant (d–f) cells. Bar, 5 μm.