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. 1998 Oct 5;143(1):135–145. doi: 10.1083/jcb.143.1.135

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Penetrance of the positioning defect in a /α cdc28-4 clb5 haploid cells. (A) Patching efficiency of a/α cdc28-4 clb5 GAL1: CLB5 and a cdc28-4 clb5 bud3 GAL1:CLB5 haploids on galactose and dextrose media. The indicated strains were patched on galactose synthetic medium and replica-plated onto dextrose or galactose synthetic medium. (a) a cdc28-4 clb5 GAL1:CLB5 [MATα], (b) a cdc28-4 clb5 GAL1:CLB5 [MATa], (c) α cdc28-4 clb5 GAL1:CLB5 [MATa], (d) α cdc28-4 clb5 GAL1:CLB5 [MATα], (e) a cdc28-4 clb5 bud3 GAL1:CLB5. Plates were photographed after 3 d at 23°C. (B) Nuclear staining of a /α cdc28-4 clb5 GAL1:CLB5 haploids. Transformants were grown in galactose synthetic medium followed by a 6-h shift to dextrose synthetic medium. DIC images were overlaid onto the corresponding DAPI images. Bar, 5 μm. (C) Nuclear staining of a cdc28-4 clb5 bud3 GAL1:CLB5 haploids. Cells were grown to early log in YEPGalactose followed by a 4-h shift to YEPDextrose. Bar, 5 μm.