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. 1998 Oct 5;143(1):135–145. doi: 10.1083/jcb.143.1.135

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Induction of GAL1: CLB5 before S phase rescues the cdc28-4 clb5 diploid lethality. (A) cdc28-4 clb5 GAL1: CLB5 diploids (MY1416) were released from a nocodazole block (YEPGalactose + 15 μg/ ml nocodazole) into YEPRaffinose. At 20-min intervals, two aliquots were removed for plating on galactose medium and cytology studies (to assess cell cycle progression in the YEPRaffinose culture). After 15 h, viability was estimated by counting the proportion of normal microcolonies emerging on YEPGalactose (open circles). Entry into S phase (open squares) was estimated from FACS analysis. At least 200 cells were scored for bud emergence (crossed squares) and nuclear positioning in the bud (crossed circles). (B) Viability on YEPGalactose. Arrows indicate examples of viable colonies. Microcolonies on a plate were photographed at the time of scoring.

Induction of GAL1: CLB5 before S phase rescues the cdc28-4 clb5 diploid lethality. (A) cdc28-4 clb5 GAL1: CLB5 diploids (MY1416) were released from a nocodazole block (YEPGalactose + 15 μg/ ml nocodazole) into YEPRaffinose. At 20-min intervals, two aliquots were removed for plating on galactose medium and cytology studies (to assess cell cycle progression in the YEPRaffinose culture). After 15 h, viability was estimated by counting the proportion of normal microcolonies emerging on YEPGalactose (open circles). Entry into S phase (open squares) was estimated from FACS analysis. At least 200 cells were scored for bud emergence (crossed squares) and nuclear positioning in the bud (crossed circles). (B) Viability on YEPGalactose. Arrows indicate examples of viable colonies. Microcolonies on a plate were photographed at the time of scoring.