Table I.

KI Treatment Selectively Removes Actin Cross-links

				% Bristles stained
Antibody		KI (M)		Uniform		None		Particulate		n
Anti-forked		0		100						71
		0.1		97		3				78
		0.2				58		42		73
		0.3				75		25		4
		0.4				78		23		26
		0.5						100		4
		0.6						100		6
Anti-singed		0		100						3
		0.1						100		31
		0.2				92		8		66
		0.3
		0.4
		0.5
		0.6				100				5

Bristles were detergent extracted, treated with KI, fixed, stained with an antibody directed against one cross-link, and then visualized by confocal microscopy. The actin bundles were either stained evenly (Uniform), not at all (None), or exhibited a very nonuniform speckled appearance (Particulate).