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. 1998 Oct 5;143(1):267–276. doi: 10.1083/jcb.143.1.267

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Inhibition of fibronectin matrix assembly with function-blocking integrin antibodies prevents stress fiber formation. Trypsinized KD fibroblasts were plated on a mixture of collagen IV and vitronectin in serum-free medium. The cells were allowed to attach and spread for 1 h before adding function-blocking antibodies directed against the β1 (P4C10, 10 μg/ml) or αvβ3 (LM609, 10 μg/ml) integrins individually or in combination. After an additional 6 h, the cells were stained for cell surface fibronectin (A) or actin cytoskeleton (B). (C) HUVECs were grown on a mixture of fibronectin and laminin in complete medium for 48 h, and were then treated with the function-blocking antibodies directed against the α5 (P1D6, 10 μg/ml) or αvβ3 (LM609, 10 μg/ml) integrins individually or in combination in serum-free medium. The cells were stained after 4 h for actin cytoskeleton.

Inhibition of fibronectin matrix assembly with function-blocking integrin antibodies prevents stress fiber formation. Trypsinized KD fibroblasts were plated on a mixture of collagen IV and vitronectin in serum-free medium. The cells were allowed to attach and spread for 1 h before adding function-blocking antibodies directed against the β1 (P4C10, 10 μg/ml) or αvβ3 (LM609, 10 μg/ml) integrins individually or in combination. After an additional 6 h, the cells were stained for cell surface fibronectin (A) or actin cytoskeleton (B). (C) HUVECs were grown on a mixture of fibronectin and laminin in complete medium for 48 h, and were then treated with the function-blocking antibodies directed against the α5 (P1D6, 10 μg/ml) or αvβ3 (LM609, 10 μg/ml) integrins individually or in combination in serum-free medium. The cells were stained after 4 h for actin cytoskeleton.

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