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Expression of a dominant negative form of Cdc42 prevents the formation of III1-C–induced filopodia. HUVECs cells were transfected GFP alone, or with GFP and Cdc42N17 before treatment with 20 μM III1-C for 4 h in serum-free medium and stained for actin cytoskeleton (top). In the lower panel, the same cells were analyzed for GFP expression to identify the transfected cells.
