Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Oct 5;143(1):225–239. doi: 10.1083/jcb.143.1.225

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Caspase inhibitors block apoptosis in S/M but not E/X extracts. (A) Inhibition of caspases blocks nuclease activity in S/M extracts but not E/X extracts. As expected, C/D extracts lack detectable nuclease activity. Experiments shown in Fig. 8 confirm that the nuclease activity is inhibitable by the specific CAD inhibitor ICAD/DFF45 (Enari et al., 1995b ; Liu et al., 1997). Lane B, buffer control. (B) Inhibition of caspases abolishes morphological apoptosis in vitro in S/M extracts but not execution phase extracts. Nuclei shown in B were selected at random. (C) Morphological changes characteristic of apoptosis occur in E/X extracts independently of caspase activity: confirmation of apoptotic morphology by electron microscopy. Arrows indicate regions of condensed chromatin. Bars: (B) 5 μm; (C) 1 μm.