Table I.

Homing of Fluorescence-labeled α6A+/+ and α6A−/− Lymphocytes to Spleen, Peripheral Lymph Nodes, and Mesenteric Lymph Nodes

		2 h						20 h
Cells		Spleen		Peripheral lymph nodes		Mesenteric lymph nodes		Peripheral and mesenteric lymph nodes
α6A+/+		3.66 ± 0.69		1.18 ± 0.12		0.97 ± 0.29		2.26
α6A−/−		3.85 ± 0.76		1.21 ± 0.45		0.74 ± 0.17		2.48

Cells were isolated from the peripheral and mesenteric lymph nodes of α6A+/+ and α6A−/− mice and labeled with either PKH2 or PKH26 as described in Materials and Methods. 3 × 10⁷ α6A+/+ and α6A−/− cells were injected into wild-type recipient mice. After 2 or 20 h, cell suspensions were isolated from the spleen, peripheral lymph nodes, and mesenteric lymph nodes of the recipient mice and subjected to FACS^® analysis to determine the percentage of α6A−/− versus α6A+/+ cells in each suspension. Values after 2 h homing are the means ± SEM obtained from a total of six recipient mice in two independent experiments. Mean for two mice are indicated after 20 h homing.