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. 1998 Oct 5;143(1):95–106. doi: 10.1083/jcb.143.1.95

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Direct interaction between mouse PKCζ and rat ASIP or C. elegans PAR-3. The recombinant GST fusion proteins shown at the top were produced in E. coli. Purified proteins were subjected to SDS-PAGE and blotted onto PVDF membranes. The blots were then probed with anti-GST antibody (left) or overlaid with recombinant mouse PKCζ, followed by immunodetection with an anti-PKCζ (ζRb2) antibody and an alkaline phosphatase–conjugated secondary antibody (right).