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. 1998 Oct 5;143(1):95–106. doi: 10.1083/jcb.143.1.95

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Colocalization of ASIP with aPKC and ZO-1 at cell junctions in MDCKII cells. Confluent MDCKII cells were doubly stained with anti-ASIP (C2) antibody (a and d, green) and anti-PKCλ (mAb) antibody (b, red) or anti-ZO-1 (e, red) followed by FITC-conjugated anti-rabbit IgG and Cy3-conjugated anti-mouse IgG antibodies. The yellow and orange staining in c and f indicates the colocalization of ASIP and PKCλ or ZO-1. Bars (c and f), 10 μm.