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. 1998 Oct 19;143(2):415–427. doi: 10.1083/jcb.143.2.415

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Deletion of imp2 causes the formation of cell filaments with defective septa and the appearance of aberrant medial ring structures. Δimp2 cells (JD141) were grown at 29°C and then shifted to 36°C for 4 h. Cells were stained with Calcofluor to visualize the septum (a), Calcofluor and DAPI to visualize the septum and the DNA (b), or rhodamine-labeled phalloidin to visualize actin (c). GFP-cdc4 was expressed in Δimp2 cells to localize medial ring structures in live cells (d, e). Δimp2 cells showed various septation defects: multinucleate compartments (b, small arrow), multiple septa between nuclei (a and b, arrowheads) and cell separation defects. Branching could also be observed (a). Abnormal actin (arrowheads in c) and contractile ring (arrowheads in d and e) structures were detected. Bar, 5 μm.