Figure 4.

Absence of an effect of mutant Drp1 on the secretory pathway. The function of the secretory pathway was monitored by cotransfecting wild-type or mutant Drp1, together with a chimera containing VSV-G tsO45 fused to GFP (Presley et al., 1997). Overexpression of mutant or wild-type Drp1 was verified by double labeling with anti-Drp1 antibody (not shown). The VSV-G chimera was accumulated in the ER by growing transfected cells at 40°C. At 0 min the cells were transferred to the permissive temperature (32°C), allowing the VSV-G chimera to exit the ER. At 15 and 30 min, most of the label was concentrated in the Golgi, and at 60 min most of the label was transferred to the plasma membrane. There was no detectable difference between cells transfected with mutant or wild-type Drp1.