Figure 6.

Absence of an effect of dominant negative mutant Drp1 on organelles other than mitochondria. Cells that overexpress mutant or wild-type Drp1 were identified by double labeling with anti-Drp1 antibody as indicated by the gray outlines. The ER was detected with an anti-VSV–G antibody by staining cells that were cotransfected with Drp1 and VSV-G carrying an ER retention signal (KKTN at the COOH terminus). The inset shows that the reticulum extends to the cell periphery, both in cells transfected with wild-type (Drp1-wt) and with mutant (Drp1-K38A). COPII-coated vesicles which bud from the ER were detected with anti-sec13 antibody. COPI-coated vesicles, which bud from the Golgi, were detected with anti-β-COP antibody. Lysosomes were detected with anti-LAMP1 antibody. Endosomes detected by cotransfecting epitope-tagged rab5 and peroxisomes detected with anti-catalase antibody were also not affected (not shown).