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. 1998 Oct 19;143(2):351–358. doi: 10.1083/jcb.143.2.351

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Effect of Drp1 on mitochondrial morphology. (A) COS-7 cells transfected with wild-type Drp1, wild-type dynamin, or mutant dynamin all show normal mitochondrial morphology. The cells were transfected with Drp1-wt (the nonneuronal isoform; 1), B-Drp1-wt (the brain-specific isoform of Drp1; 2), Dyn1-wt (dynamin I wild-type; 3) or Dyn1-K44A (dynamin I K44A, which blocks endocytosis [van der Bliek et al., 1993]; 4). The cells were double-labeled with a mitochondrial antibody (anti-COXI) and either anti-Drp1 or anti-dynamin antibody. Outlines of cells that are overexpressing Drp1 or dynamin are superimposed on the mitochondrial staining. Untransfected cells were not outlined. (B) COS-7 cells transfected with mutant Drp1 show collapsed mitochondria. Two examples of each condition are shown. The following constructs were tested: Drp1-K38A (the K38A mutant of the non-neuronal isoform; 5 and 5′), and B-Drp1-K38A (the K38A mutant of the brain specific isoform; 6 and 6′).