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. 1998 Oct 19;143(2):283–295. doi: 10.1083/jcb.143.2.283

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(A) Excess Xmad2 sustains Cdc2 activity by maintaining cyclin B levels in the absence of any nuclei and nocodazole. The addition of Xmad2 (100 ng/μl of extract), sperm nuclei (500/μl of extract), and/or nocodazole to CSF-arrested extracts is indicated on the left. Samples were taken for H1 kinase measurement and for cyclin B Western blots at the indicated times after calcium addition. (B) Excess Xmad2 arrests cycling extracts at the first mitosis. Eggs were activated in vitro to start the embryonic cell cycle and extracts were prepared. This cycling extract entered mitosis at 30 and 90 min upon incubation at 23°C (top) and became arrested at first mitosis in the presence of excess 6H-Xmad2 (bottom). Autoradiograms of H1 kinase assays are shown.