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. 1998 Oct 19;143(2):319–331. doi: 10.1083/jcb.143.2.319

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TAP/p115 mutants used in transfection studies. (A) Diagram of analyzed constructs. Light gray rectangles, Uso1p homology (H1, H2, and H3) regions. Dark gray rectangles, coiled-coil domains (C1–C4). Black rectangle, acidic domain (A). (B) Lysates of COS-7 cells tranfected with specific TAP/p115 constructs were analyzed by immunoblotting. Recombinant proteins of the appropriate molecular weight (∼108 kD for TAP-FL; ∼86 kD for TAP-C764, and ∼75 kD for TAP-N283) were detected.