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. 1998 Oct 19;143(2):333–349. doi: 10.1083/jcb.143.2.333

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Kinetics of FM 4-64 internalization. DNM1 (JSY1238) and dnm1Δ (JSY1361) cells grown in YPD medium were labeled with FM 4-64 for 30 min at 0°C, washed, and chased in fresh medium at 30°C for 60 min. Aliquots removed at 0 (A and B), 10 (C and D), 20 (E and F), 40 (G and H), and 60 (I and J) min were scored for FM 4-64 distribution (see Table II) and photographed. Representative differential interference contrast (A, C, E, G, and I) and fluorescence (B, D, F, H, and J) images are shown for the dnm1Δ strain and were identical to those observed in wild type. Bar, 2 μm.