Table II.
Kinetics of FM 4-64 Internalization
| FM 4-64 Localization | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Time | Strain | PM | PM + E | E + V | V | |||||
| min | ||||||||||
| 0* | WT | 50/50 (100%) | 0/50 (0%) | 0/50 (0%) | 0/50 (0%) | |||||
| dnm1Δ | 50/50 (100%) | 0/50 (0%) | 0/50 (0%) | 0/50 (0%) | ||||||
| 10 | WT | 0/68 (0%) | 68/68 (100%) | 0/68 (0%) | 0/68 (0%) | |||||
| dnm1Δ | 0/55 (0%) | 55/55 (100%) | 0/55 (0%) | 0/55 (0%) | ||||||
| 20 | WT | 0/67 (0%) | 67/67 (100%) | 0/67 (0%) | 0/67 (0%) | |||||
| dnm1Δ | 0/58 (0%) | 58/58 (100%) | 0/58 (0%) | 0/58 (0%) | ||||||
| 40 | WT | 0/56 (0%) | 0/56 (0%) | 56/56 (100%) | 0/56 (0%) | |||||
| dnm1Δ | 0/50 (0%) | 0/50 (0%) | 50/50 (100%) | 0/50 (0%) | ||||||
| 60 | WT | 0/57 (0%) | 0/57 (0%) | 0/57 (0%) | 57/57 (100%) | |||||
| dnm1Δ | 0/60 (0%) | 0/60 (0%) | 0/60 (0%) | 60/60 (100%) | ||||||
PM, plasma membrane; E, endosome-like intermediate; V, vacuole.
*
0 min time point scored after incubation and wash performed at 0°C. Remaining time points were scored during the 30°C chase (see text).