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. 1998 Oct 19;143(2):309–318. doi: 10.1083/jcb.143.2.309

Figure 5.

Figure 5

Analysis of CAS binding to Imp α2 in vitro. Recombinant wild-type or M2 mutant Imp α2 proteins were coupled to agarose beads and used to construct microaffinity columns. Recombinant, NH2 terminally His-tagged CAS protein was preincubated on ice with Ran and GTP before loading onto the columns either directly or after addition of a 100-fold molar excess of a wild-type or defective mutant T NLS peptide. After extensive washing, bound proteins were eluted from the affinity columns and analyzed by SDS-PAGE.

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