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. 1998 Oct 19;143(2):309–318. doi: 10.1083/jcb.143.2.309

Table I.

Interaction of Imp α2 with Various Cellular Targets Measured by Yeast Two-hybrid Analysis

DNA-binding domain hybrid β-gal activity (mOD/ml)*
VP16 VP16/Imp α2
GAL4 5       7
GAL4/Imp β 3       8
GAL4/Imp βΔ1/261 4 1,198
GAL4/CAS 3     434
GAL4/T-NLS 4 1,211
GAL4/LEF-1 (297–399) 4     130
*

Data are given as milli optical density units per ml of yeast extract observed in yeast transformants expressing the indicated GAL4 and VP16 fusion proteins. Induced β-gal activities were determined as described previously (Bogerd et al., 1995) and are representative of values obtained in three independent experiments.  

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