Table I.
Interaction of Imp α2 with Various Cellular Targets Measured by Yeast Two-hybrid Analysis
DNA-binding domain hybrid | β-gal activity (mOD/ml)* | |||
---|---|---|---|---|
VP16 | VP16/Imp α2 | |||
GAL4 | 5 | 7 | ||
GAL4/Imp β | 3 | 8 | ||
GAL4/Imp βΔ1/261 | 4 | 1,198 | ||
GAL4/CAS | 3 | 434 | ||
GAL4/T-NLS | 4 | 1,211 | ||
GAL4/LEF-1 (297–399) | 4 | 130 |
Data are given as milli optical density units per ml of yeast extract observed in yeast transformants expressing the indicated GAL4 and VP16 fusion proteins. Induced β-gal activities were determined as described previously (Bogerd et al., 1995) and are representative of values obtained in three independent experiments.