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. 1998 Oct 19;143(2):309–318. doi: 10.1083/jcb.143.2.309

Table IV.

Interaction of Imp a2 Mutants with CAS and Imp β Measured by Mammalian Two-hybrid analysis

VP16 activation domain hybrid Relative CAT activity
GAL4/CAS GAL4/Imp βΔ1/261
Imp α2/VP16 ++ ++
α2Δ501/529/VP16 ++ ++
α2Δ491/529/VP16 +
α2Δ136/249/VP16 + ++
α2Δ136/365/VP16 +/− ++
α2Δ136/382/VP16 ++
α2Δ136/406/VP16 ++
α2M1/VP16 ++ ++
α2M2/VP16 +
α2M3/VP16 ++

Protein interaction data were determined by mammalian two-hybrid analysis in transfected human 293T cells, as described in Fig. 3. Activity is given relative to the level seen with the wild-type Imp α2 fusion protein as follows: ++, >40% of wild-type; +, 14–40% of wild-type; +/−, 1–5% of wild-type; −, <0.2% of wild-type.  

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