Table IV.
Interaction of Imp a2 Mutants with CAS and Imp β Measured by Mammalian Two-hybrid analysis
VP16 activation domain hybrid | Relative CAT activity | |||
---|---|---|---|---|
GAL4/CAS | GAL4/Imp βΔ1/261 | |||
Imp α2/VP16 | ++ | ++ | ||
α2Δ501/529/VP16 | ++ | ++ | ||
α2Δ491/529/VP16 | − | + | ||
α2Δ136/249/VP16 | + | ++ | ||
α2Δ136/365/VP16 | +/− | ++ | ||
α2Δ136/382/VP16 | − | ++ | ||
α2Δ136/406/VP16 | − | ++ | ||
α2M1/VP16 | ++ | ++ | ||
α2M2/VP16 | − | + | ||
α2M3/VP16 | − | ++ |
Protein interaction data were determined by mammalian two-hybrid analysis in transfected human 293T cells, as described in Fig. 3. Activity is given relative to the level seen with the wild-type Imp α2 fusion protein as follows: ++, >40% of wild-type; +, 14–40% of wild-type; +/−, 1–5% of wild-type; −, <0.2% of wild-type.