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. 1998 Oct 19;143(2):375–389. doi: 10.1083/jcb.143.2.375

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Northern analysis of KEL1 and FUS1 mRNA. mRNA was prepared as described in Materials and Methods from a /α wild-type (JP54), α wild-type (IH3197), a wild-type (IH3196), a kel1Δ (JP358), and a fus1Δ (JP52) strains after cells were treated with α-factor for 20 min (+, treated; −, untreated). Northern blots were hybridized with a probe to KEL1 (top) or FUS1 (third panel). Blots were stripped and hybridized with a probe to TCM1 to serve as a loading control.