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. 1998 Oct 19;143(2):375–389. doi: 10.1083/jcb.143.2.375

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Kel1p and Kel2p are involved in mating and morphology. (A) kel1Δ mutants are defective in mating. kel1Δ strain JP363 was transformed with a CEN-ARS plasmid containing KEL1 (pJP207), a 2μ plasmid expressing Kel2p (pJP92), or vector (YCplac111). The wild-type control (IH3196) was transformed with vector (YCplac111). Strains were mated to an α fus1 fus2 strain (IH2351) as described in Materials and Methods. (B) Wild-type (a–d) and kel1Δ (JP363; e–h) mutants were mated on filters to wild-type strain IH2350 carrying the RAS2-GFP fusion plasmid (pJW192) as described in Materials and Methods. Nuclei were visualized by DAPI staining (d and h). RAS2-GFP was visualized by fluorescence microscopy (b and f). (C) kel1Δ and kel1Δ kel2Δ mutants exhibit altered morphology. Morphology of isogenic (a) wild-type (IH3196), (b) kel1Δ (JP363), (c) kel2Δ (JP371), and (d) kel1Δ kel2Δ (JP385) strains. (D) Morphological phenotype exhibited by cells overexpressing KEL1 or KEL2. Morphology exhibited by a wild-type strain (IH3196) transformed with (a) vector (YEp351), (b) 2μ KEL1 (pJP81), (c) vector (pRS426), or (d) pGAL KEL2 (pJP160) grown in SD-Ura (a and b) or S galactose-Ura (c and d).