Table I.
Yeast Strains and Plasmids
| Strains | Genotype | Source | ||
|---|---|---|---|---|
| IH2350* | MATα ura3-52 his4-34 trp1Δ1 | IH collection | ||
| IH2351* | MATα ura3-52 trp1Δ1 fus1Δ1 fus2Δ3 | IH collection | ||
| IH1783** | MAT a | IH collection | ||
| IH3077** | MATa mpk1Δ::TRP1 | IH collection | ||
| IH3196 | MATa leu2Δ1 | Philips and Herskowitz (1997) | ||
| IH3197 | MATα leu2Δ1 | IH collection | ||
| IH3204 | MATa spa2Δ::TRP1 trp1Δ99 leu2Δ1 | IH collection | ||
| JP52 | MATa fus1Δ::TRP1 trp1Δ99 | Philips and Herskowitz (1997) | ||
| JP54 | MATa/MATα | This study | ||
| JP147 | MATa fps1Δ::URA3 leu2Δ1 | Philips and Herskowitz (1997) | ||
| JP317 | MATa PKC1::PKC1-R398P-URA3 leu2Δ1 | Philips and Herskowitz (1997) | ||
| JP338 | MATa ura3::URA3 leu2Δ1 | This study | ||
| JP358 | MATa kel1Δ::hisG-URA3-hisG leu2Δ1 | This study | ||
| JP363 | MATa kel1Δ::hisG leu2Δ1 | This study | ||
| JP371 | MATa kel2Δ::LEU2 leu2Δ1 | This study | ||
| JP385 | MATa kel1Δ::hisG kel2Δ::LEU2 leu2Δ1 | This study | ||
| JP410 | MATa kel1Δ::hisG-URA3-hisG fus1Δ::TRP1 trp1Δ99 | This study | ||
| JP412 | MATa kel2Δ::hisG-URA3-hisG fus1Δ::TRP1 trp1Δ99 | This study | ||
| JP414 | MATa fps1Δ::URA3 kel1Δ::hisG leu2Δ1 | This study | ||
| JP415 | MATa fps1Δ::URA3 kel2Δ::LEU2 leu2Δ1 | This study | ||
| JP416 | MATa fus2Δ::URA3 kel2Δ::LEU2 leu2Δ1 | This study | ||
| JP417 | MATa fus2Δ::URA3 kel1Δ::hisG leu2Δ1 | This study | ||
| JP418 | MATa fus2Δ::URA3 leu2Δ1 | This study | ||
| JP445A** | MATα kel1Δ::hisG-URA3-hisG | This study | ||
| JP446A** | MATα kel2Δ::hisG-URA3-hisG | This study | ||
| JP490** | MATa mpk1Δ::TRP1 kel1Δ::hisG-URA3-hisG | This study | ||
| JP491** | MATα mpk1Δ::TRP1 kel2Δ::hisG-URA3-hisG | This study | ||
| JP500 | MATa fps1Δ::URA3 kel1Δ::hisG kel2Δ::LEU2 leu2Δ1 | This study | ||
| JP502 | MATa spa2Δ::TRP1 kel1Δ::hisG-URA3-hisG trp1Δ99 leu2Δ1 | This study | ||
| Plasmid name | Description | |||
| pJP2 | fus1Δ::TRP1 | Philips and Herskowitz (1997) | ||
| pJP52 | fps1Δ::URA3 | Philips and Herskowitz (1997) | ||
| pJP67 (YCp50-DS1) | YCp50-PKC1-R398P | Nonaka et al. (1995) | ||
| pJP72 | pRS306-PKC1-R398P | Philips and Herskowitz (1997) | ||
| pJP81 | YEp351-KEL1 | This study | ||
| pJP92 | YEp351-KEL2 | This study | ||
| pJP94 | kel1Δ::hisG-URA3-hisG | This study | ||
| pJP113 | kel2Δ::LEU2 | This study | ||
| pJP123 | YEp351-KEL2 | This study | ||
| pJP126 | YEp351-KEL2-GFP | This study | ||
| pJP127 | YEp351-KEL1 | This study | ||
| pJP129 | YEp351-KEL1-GFP | This study | ||
| pJP131 | YEp351-KEL2-HA | This study | ||
| pJP138 | kel2Δ::hisG-URA3-hisG | This study | ||
| pJP139 | YIp5-KEL1-GFP | This study | ||
| pJP143 | YIp5-KEL1 | This study | ||
| pJP158 | pEG202-KEL2-DBD | This study | ||
| pJP160 | pRS426-GAL-KEL2 | This study | ||
| pJP167 | pJG4-5-KEL1-AD | This study | ||
| pJP168 | pJG4-5-KEL2-AD | This study | ||
| pJP202 | YEp351-KEL1-HA | This study | ||
| pJP207 | YCplac111-KEL1 | This study | ||
| pJP209 | YCplac111-KEL1-HA | This study | ||
| pJW192 | RAS2-GFP | J. Whistler | ||
| pKOFUS2 | fus2Δ::URA3 | Philips and Herskowitz (1997) | ||
| pRFHM1 | Bicoid-DBD | Gyuris et al. (1993) | ||
| pSH18-34 | LexAop-LacZ | Gyuris et al. (1993) | ||
| pRS306 | ||||
| YCplac111 | ||||
| YEp351 | ||||
| YIp5 | ||||
| pJG4-5 | ||||
| pRS426 |
pRS306, pRS426, YEp351, and YIp5 are described in Guthrie and Fink (1991). pEG202 and pJG4-5 are described in Gyuris et al. (1993). YCplac111 is described in Gietz and Sugino (1988).
*
Isogenic to IH2350.
**
Isogenic derivatives in the EG123 strain background, whose full genotype is trp1 ura3 his4 leu2 can1. All other strains are isogenic derivatives of IH3160, whose full genotype is MATa ade2-101 ura3-52 met1-1 HMLa HMRa.