Table II.

The Effect of KEL1 Overexpression on Cell Fusion in spa2Δ, fps1Δ, and PKC1-R398P Mutants

Genotype		Plasmid		Prezygotes*
				%
WT		vector		4.7 ± 1.1
PKC1-R398P		vector		51 ± 0.5
PKC1-R398P		KEL1		29 ± 7
WT		vector		2.3 ± 0.4
spa2Δ		vector		42 ± 2
spa2Δ		KEL1		25 ± 4
fps1Δ		vector		84 ± 3
fps1Δ		KEL1		67 ± 3

For lines 1–3, strains were: WT (JP338) and PKC1-R398P (JP317). Strains were transformed with YEp351 containing KEL1 (pJP81) or YEp351 alone as indicated. For lines 4–8, strains were: WT (IH3196), spa2Δ (IH3204), and fps1Δ (JP147). For lines 1–3, strains were grown overnight in SD-Leu Ura. For lines 4–8, strains were grown overnight in SD-Leu. All strains were mated to a wild-type α strain (IH2350). At least 100 partnered cells were counted in each experiment to determine % prezygotes. Values are means of three experiments ± SD.  

^*Percent  prezygotes represents the number of prezygotes/(zygotes + prezygotes).