Table III.
The Effect of Deletion of KEL1 and KEL2 on Cell Fusion
| Genotype | Prezygotes* | |
|---|---|---|
| % | ||
| WT | 2.6 ± 0.7 | |
| kel1Δ | 9.4 ± 1.9 | |
| kel2Δ | 3.7 ± 0.8 | |
| kel1Δ kel2Δ | 7.1 ± 1.4 | |
| fus1Δ | 22.4 ± 3.8 | |
| fus1Δ kel1Δ | 54.0 ± 10.6 | |
| fus1Δ kel2Δ | 31.9 ± 3.5 | |
| fus2Δ | 44 ± 7 | |
| fus2Δ kel1Δ | 70 ± 3 | |
| fus2Δ kel2Δ | 38 ± 2 | |
| fps1Δ | 62 ± 7 | |
| fps1Δ kel1Δ | 93 ± 4 | |
| fps1Δ kel2Δ | 66 ± 13 | |
| fps1Δ kel1Δ kel2Δ | 99.3 ± 0.2 | |
| spa2Δ | 39 ± 12 | |
| spa2Δ kel1Δ | 42 ± 10 |
Analysis of lines 1–7, 8–10, 11–14, and 15–16 were carried out separately, in each case with wild-type and kel1Δ controls that behaved similarly to lines 1 and 2, respectively. Strains were WT (IH3196), kel1Δ (JP363), kel2Δ (JP371), fus1Δ (JP52), fus1Δ kel1Δ (JP410), fus1Δ kel2Δ (JP412), kel1Δ kel2Δ (JP385), fus2Δ (JP418), fus2Δ kel1Δ (JP417), fus2Δ kel2Δ (JP416), fps1Δ (JP147), fps1Δ kel1Δ (JP414), fps1Δ kel2Δ (JP415), fps1Δ kel1Δ kel2Δ (JP500), spa2Δ (IH3204), and spa2Δ kel1Δ (JP502). Cells were grown in YEPD and were mated to a wild-type α strain (IH2350). For lines 1–7, values are means of at least four experiments in which a total of at least 900 partnered cells were counted ± SD. For lines 8–14, values are the means of three experiments in which more than 100 partnered cells were counted per experiment. For lines 15 and 16, more than 200 partnered cells were counted in three experiments.
Percent prezygotes represents the number of prezygotes/(zygotes + prezytotes).