Table IV.

The Effect of PKC1-R398P on Cell Fusion in kel1Δ and kel2Δ Mutants

Genotype		Prezygotes*
		Vector		PKC1-R398P
WT		5.5 ± 1.0		47.9 ± 2.6
kel1Δ		30.2 ± 2.4		79.2 ± 0.3
kel2Δ		8.7 ± 2.8		53.9 ± 4.8
fus1Δ		47.3 ± 3.7		87.6 ± 3.6
kel1Δ kel2Δ		19.9 ± 2.0		69.6 ± 1.8

Strains were: WT (IH3196), kel1Δ (JP363), kel2Δ (JP371), fus1Δ (JP52), and kel1Δ kel2Δ (JP385). Strains were transformed with either YCp50 containing PKC1-R398P (pJP67) or YCp50 alone as indicated. Strains were grown in SD-Ura, and were mated to a wild-type α strain (IH2350). More than 200 partnered cells were counted in each experiment to determine % prezygotes. Values are means of three experiments ± SD.  

^* Percent prezygotes represents the number of prezygotes/(zygotes + prezygotes).