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. 1998 Aug 24;142(4):923–936. doi: 10.1083/jcb.142.4.923

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Internalized anti-Tac colocalizes with furin after a 60-min chase. Cells were incubated with 4 mg/ml anti-Tac monoclonal antibodies conjugated to Alexa 488 fluorescent dye (a and c) for 10 min. Cells were fixed immediately (a and b) or chased in medium for 60 min (c and d). After fixation, cells were permeabilized and stained with polyclonal antibodies against furin (b and d) followed by rhodamine-conjugated goat anti–rabbit secondary antibodies. In the absence of a chase, anti-Tac was detected in a distribution that resembles the endocytic recycling compartment, and is surrounded by the TGN (arrows) as labeled by anti-furin antibodies. With a 60-min chase, anti-Tac predominantly colocalized with furin (arrows). Bar, 5 μm.