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. 1998 Aug 24;142(4):975–988. doi: 10.1083/jcb.142.4.975

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Transient mitochondrial depolarizations require mitochondrial calcium uptake. (a) After microinjection of a cell with DAPPAC and fura-2–free acid, a challenge with caffeine (10 mM) raised [Ca²⁺]_i and caused a twitch, showing that the SR calcium release mechanism was operational. Nevertheless, the mitochondrial flicker was almost completely suppressed, as shown in the surface and line images shown in c (upper and lower traces, respectively), and compared with a control obtained in the same preparation (b, upper and lower panels). To illustrate the time course of cell shortening, a binary image of the cell was created. The intensity was measured in a region that included the edge of the cell. Since the signal is simply a function of the number of pixels within the region set to unity, cell shortening reduced the signal, illustrating the time course of the twitch.

Transient mitochondrial depolarizations require mitochondrial calcium uptake. (a) After microinjection of a cell with DAPPAC and fura-2–free acid, a challenge with caffeine (10 mM) raised [Ca²⁺]_i and caused a twitch, showing that the SR calcium release mechanism was operational. Nevertheless, the mitochondrial flicker was almost completely suppressed, as shown in the surface and line images shown in c (upper and lower traces, respectively), and compared with a control obtained in the same preparation (b, upper and lower panels). To illustrate the time course of cell shortening, a binary image of the cell was created. The intensity was measured in a region that included the edge of the cell. Since the signal is simply a function of the number of pixels within the region set to unity, cell shortening reduced the signal, illustrating the time course of the twitch.