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. 1999 Jan 25;144(2):201–211. doi: 10.1083/jcb.144.2.201

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CPRF2 is imported into the nucleus of evacuolated parsley protoplasts in response to irradiation with UV-containing white light. Autoradiograms of EMSSAs with a radioactive-labeled C-box probe are shown. For EMSSA 50 μg per lane of cytosolic (panel I) and 20 μg per lane of nuclear (panel II) extracts isolated from dark-cultivated cells were used that were either further kept in continuous darkness (A) or irradiated for 30 min with UV-containing white light (B) before compartment separation. For CPRF–antiserum interaction tests, the extracts were incubated for 10 min on ice with 1 μl of serum and the radioactive-labeled C-box probe before loading the samples on the gel (A and B, lanes 3 and 4, and 6 and 7, respectively). In lanes 2 and 6, no serum was added (−). DNA–CPRF2 complexes are marked (F2). Arrows, positions of supershifted DNA–CPRF2 complex. For further abbreviations see Fig. 1.