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. 1999 Jan 25;144(2):201–211. doi: 10.1083/jcb.144.2.201

Table I.

Test of Different Polyclonal Antisera Raised against rCPRF1, rCPRF2, and rCPRF4 for Cross-reactivity in an ELISA

Antiserum Antigen*
rCPRF1 rCPRF2 rCPRF4
Anti-CPRF1 100§   1  46
Anti-CPRF2        6 100   5
Anti-CPRF4   22   5 100
*

 Highly purified, recombinant and refolded (His)6-tagged CPRF1, CPRF2, and CPRF4 were used as antigens.  

 Antisera were produced in mice as described in Kircher et al. (1998).  

§

 The ELISA signal obtained from the antiserum incubated with the corresponding antigen was set to 100. The relative signals of the preimmunosera were between 0 and 5 (data not shown).