Table I.
Antiserum‡ | Antigen* | |||||
---|---|---|---|---|---|---|
rCPRF1 | rCPRF2 | rCPRF4 | ||||
Anti-CPRF1 | 100§ | 1 | 46 | |||
Anti-CPRF2 | 6 | 100 | 5 | |||
Anti-CPRF4 | 22 | 5 | 100 |
Highly purified, recombinant and refolded (His)6-tagged CPRF1, CPRF2, and CPRF4 were used as antigens.
Antisera were produced in mice as described in Kircher et al. (1998).
The ELISA signal obtained from the antiserum incubated with the corresponding antigen was set to 100. The relative signals of the preimmunosera were between 0 and 5 (data not shown).