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. 1999 Jan 25;144(2):267–279. doi: 10.1083/jcb.144.2.267

Figure 2.

Figure 2

Western blots of vv proteins analyzed under reducing and nonreducing conditions. In A analysis of four IMV membrane proteins, p21 (A17L), p16 (A14L), p35 (H3R), and p32 (D8L). Cell lysates of infected HeLa cells treated (F) or not treated (L) with rifampicin or purified virus (V) were run on 15% SDS-PAGE. Before electrophoresis the samples were boiled for 3 min in LSB with (R) or without (NR) 5% β-ME and 100 mM DTT. The proteins were detected by Western blotting using the respective antibodies. On the right side of the figure the positions of the 54-, 38-, and 34-kD marker proteins is indicated. In B, three vv core proteins 4a (A10L), p39 (A4L), and p25 (L4R) were analyzed as described in A. Note that in rifampicin-blocked cell lysates for both 4a and p25 only their precursor form (of 110 and 28 kD, respectively) can be detected that is subsequently cleaved to the 65- and 25-kD mature forms, respectively, in the IMV. Some of the uncleaved form, can however, still be detected in the isolated virions. The positions of the 130-, 66-, 54-, 38-, and 34-kD marker proteins are indicated on the right.