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. 1999 Jan 25;144(2):267–279. doi: 10.1083/jcb.144.2.267

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In the presence of DTT apparently normal looking IMVs are formed but in some of them the viral membranes detach from the underlying core. A shows Epon sections of control, untreated HeLa cells infected with vv for 8 h. IMVs and immature viruses (IVs) are evident. In the inset on the left the outer and inner membrane profiles of the IMV are indicated by arrows. The inset on the right shows an intermediate stage between the IV and IMV (arrowhead) next to a IV. B and C show vv-infected cells treated with 5 mM DTT from 4 h after infection onwards and fixed at 8 h after infection (see Results for details). In B, one sees both normal looking IMVs (star) as well as aberrant IMVs (arrowheads), where the viral core is seen as a distinct entity separated from the outer membranes. This is more evident at higher magnifications in C–F; in C a normal IV is apparent. The arrowheads indicate the viral core that in all these examples appears as a distinct entity. Bars, 100 nm.