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. 1999 Jan 25;144(2):267–279. doi: 10.1083/jcb.144.2.267

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Negative staining EM of particles isolated from untreated (B) or DTT treated cells (A, C, and D). Particles from infected HeLa cells untreated or treated with DTT were isolated as described in Materials and Methods. The particles were absorbed to 300 mesh formvar and carbon-coated grids and immuno-labeled with antibodies to p39 (A, B, and D) and to p16 (C), followed by protein A–gold. Whereas virions isolated from untreated cells are not labeled with p39 (B), the particles from treated cells label heavily with the antibody (A and D). The anti-p16 antibody labels fragments that are still attached to the particles isolated from DTT-treated cells. These fragments often appear as tubular structures (C). Bars, 100 nm.