Table III.
Infectivity of vv Isolated from DTT-treated or Control Cells Determined by Indirect Immunofluorescence*
| MOI‡ | DNA positive§ | IF positive‖ | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5 | 10 | 20 | 5 | 10 | 20 | |||||||
| Control ¶ | 98% | 100% | 100% | 90% | 100% | 100% | ||||||
| DTT** | 27% | 34% | 52% | 2% | 11% | 12% | ||||||
HeLa cells grown on coverslips were infected with different MOIs for 60 min at 37°C and fixed at 6 h after infection. The infectivity is expressed as the percentage of a total of 50 cells that labeled positive for viral DNA or viral late proteins.
To calculate the MOI, the virus-titer was calculated as PFU/ml.
Fixed cells were stained with Hoechst and scored positive if clear cytoplasmic DNA labeling typical of viral DNA was observed.
Fixed cells were labeled with a rabbit antibody to the viral core proteins and with donkey anti-rabbit coupled to FITC and scored positive if typical viral factories in the perinuclear region could be detected. IF, indirect immunofluorescence.
IMV isolated from untreated cells.
IMV isolated from DTT-treated cells.