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. 1999 Jan 25;144(2):305–313. doi: 10.1083/jcb.144.2.305

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Effects of microtubule depolymerization on C3-induced cleavage. NRK cells were microinjected with C3 during metaphase followed by treatment with 2.5 μM nocodazole. Time in minutes after the addition (t = 0) of nocodazole is located in the upper right corner. When cells were treated with nocodazole after anaphase onset or at the initiation of cytokinesis (a–e) the furrow continued to ingress. Those locked in metaphase with nocodazole treatment (f–j) underwent cortical ingression without entering anaphase (g and h). This cell eventually divided into a daughter cell containing all of the chromosomes and an anuclear fragment (i and j, arrows). Bar, 20 μm.