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. 1999 Feb 8;144(3):389–401. doi: 10.1083/jcb.144.3.389

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Nuclear accumulation of L25-GFP in nucleoporin mutants. Analysis of L25-GFP nuclear accumulation in nucleoporin and ribosome assembly mutants. Haploid double mutants (see Table I) nup49-313/L25-GFP, nsp1-ala6/L25-GFP nup85::HIS3/ L25-GFP, nup84::HIS3/L25-GFP, and nop1-7/L25-GFP were grown as described in Materials and Methods in YPD-medium to OD (600 nm) of 0.2 OD before shift for 14 h to 33°C and reshift for further 4 h to 20°C. Cells were viewed in the fluorescence microscope to see L25-GFP and under Nomarski optics.