Table II.
Two-Hybrid Interactions of mBPAG-C1 and NFTPs in the Yeast Strain HF7c
| GBD-mBPAG-C1 | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAD- | L | M | H | L + M | L + H | LΔ | MΔ | HΔ | LΔ + MΔ | LΔ + HΔ | ||||||||||
| HIS+ | − | − | − | − | − | SC | SC | SC | SC | SC + FC | ||||||||||
| LacZ+ | ND | ND | ND | ND | ND | − | − | − | − | + | ||||||||||
HF7c cells cotransformed with pGBT-mBPAG-C1 and GAD-fused NFTPs constructs were selected in minus Trp-Leu-His media for 7 d. SC and FC correspond to the appearance of slow growing and fast growing colonies, respectively; minus sign, no colonies. β-Galactosidase filter lift assays were also performed for the growing colonies; plus and minus signs represented the results of the filter assays; ND, not determined. L, M, H, LΔ, MΔ, and HΔ refer to NFL24-542, NFM, NFH, NFL1-415, NFM1-421, and NFH1-415, respectively. GAD-NFL24-542, GAD-NFM, and GAD-NFH were expressed from the higher expression vector pACT2, whereas GAD-fused tailless NFTPs were expressed from pGAD424 vector.