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. 1999 Feb 8;144(3):563–574. doi: 10.1083/jcb.144.3.563

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Frozen human thymus section stained with anti-laminin 5 α3 chain mAb BM165. (a and b) BM165 staining on vessels; staining was revealed by FITC conjugated goat anti–mouse F(ab)′₂ fragments and analysis was performed by laser scanning confocal microscopy. Cell nuclei were stained with propidium iodide. (c and d) BM165 staining on stellate thymic epithelial cells; double staining obtained with mAb BM165 revealed by rabbit anti– mouse IgG1 F(ab)′₂ fragments followed by PE conjugated goat anti–rabbit F(ab)′₂ fragments, and the anti-keratin CK19 revealed by FITC conjugated goat anti–mouse IgM Ab. Controls made with secondary Abs gave no staining (not shown).