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The putative appendage domain of β3 subunits of AP3 complex inhibits SV production from plasma membrane but not that from endosome. Standard in vitro budding reactions were performed with 4°C-labeled (PM) or 15°C-labeled (ES), Percoll-washed N49A/PC12 membranes in the presence or absence of 150 μM GST fusion proteins bearing appendage domain of β3A, β3B, or β3Amt. The amount of SV production was normalized to the control reaction incubated with added 150 μM GST.
