Table II.
Clones Derived from Individually Fished Cells
| Starting cell lines | Feeder layers | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E14 | EF | E14 ep. | ||||||||||||||||
| PE | ep. | pal. | PE | ep. | pal. | PE | ep. | pal. | ||||||||||
| E14 | 59 | 871 * | 122 | 32 | 29 | 712 | 46 | 50 | 502 | |||||||||
| (16/27) | (14) | (2) | (7/22) | (2) | (5) | (12/26) | (6) | (6) | ||||||||||
| E14 pal. | 71 | 85 | 15 | 41 | 221 | 781 | 56 | 601 | 403 | |||||||||
| (20/28) | (17) | (3) | (9/22) | (2) | (7) | (15/27) | (9) | (6) | ||||||||||
| E14 ep. | 82 | 100 | 0 | 45 | 100 | 0 | 45 | 100 | 0 | |||||||||
| (9/11) | (9) | (0) | (5/11) | (5) | (0) | (5/11) | (5) | (0) | ||||||||||
The table summarizes the results of 15 individual fished cell cloning operations. All the values given are percents, except for the numbers in italics and parentheses, which give the total number of clones observed over the number of individually plated cells, and the total number of epithelial or palmate colonies obtained. The E14 line was analyzed at passages 8 and 16; E14 pal. at passages 2 and 6. No differences were observed and averages are presented. PE, plating efficiency; ep., epithelial; pal., palmate; EF, embryonic fibroblasts.
*
The exponents refer to the numbers of clones from fished cells that were established in culture and analyzed (see Table III).