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MT cosedimentation of the HC deletion mutants expressed alone or together with LC. Taxol-stabilized MTs and AMP-PNP were added to lysates from COS cells expressing HC (H) or the deletion mutants (H682, H810, and H891) alone or together with LC (H+L, H682+L, H810+L, and H891+L). MTs and bound proteins were sedimented through a sucrose cushion. The MT pellets (P) and supernatants (S) were immunoblotted to detect the expressed proteins using polyclonal antibodies to the myc- and HA-tags.
