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. 1998 Nov 16;143(4):973–990. doi: 10.1083/jcb.143.4.973

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At 19.5°C, the γ-adaptin subunit of AP-1–type clathrin coats is relocalized from the TGN to EE/RE. Fluorophore-labeled B-fragment (A and C) was internalized for 1 h into HeLa cells at 19.5°C. The cells were then either fixed directly (A and B), or shifted for 30 min to 37°C (C and D) before fixation and staining for γ-adaptin (B and D). Note that at 19.5°C, γ-adaptin and B-fragment colocalized on EE/RE (A and B), and after shift to 37°C, γ-adaptin was relocalized to the TGN, in parallel with B-fragment accumulation in Golgi cisternae (C and D).