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. 1998 Nov 16;143(4):973–990. doi: 10.1083/jcb.143.4.973

Figure 9.

Figure 9

γ-Adaptin and clathrin colocalize with B-fragment and Tf-HRP at the ultrastructural level on coated membrane profiles of EE/RE. (A–E) HeLa cells were incubated for 1 h with B-fragment and BSA-gold (5-nm gold particles) at 19.5°C. Cryosections of these cells were labeled with anti–B-fragment antibody (15-nm gold particles) and anti–γ-adaptin antibody (10-nm gold particles in A–C) or anti-clathrin antibody (10-nm gold particles in D and E). In A–C, arrowheads indicate regions of colocalization between γ-adaptin and B-fragment. In D and E, arrowheads point out regions of colocalization between clathrin and B-fragment. (F-I) Serum-starved HeLa cells were incubated for 1 h with B-fragment and Tf-HRP at 19.5°C. Cryosections of these cells were labeled with anti–B-fragment antibody (15-nm gold particles), anti-HRP antibody (10-nm gold particles), and anti–γ-adaptin antibody (5-nm gold particles). Arrowheads in F and G point to double- or triple-labeled profiles. (H and I) Magnification of selected structures. Bars, 100 nm.