Table I.

Localization of Marker Proteins in γ-Adaptin–positive Membrane Profiles

Staining of γ-adaptin versus		Colocalization
		%
B-fragment		9.7 (± 0.62; n = 5)
Tf-HRP		13.2 (± 3.1; n = 4)
BSA-gold		0.27 (n = 1)

Quantification was done as described in Materials and Methods on cryosections of HeLa cells that had internalized BSA-gold and B-fragment or Tf-HRP and B-fragment for 1 h at 19.5°C. The percentage of marker protein–specific gold label in γ-adaptin–positive membrane profiles is given (± SE; n, the number of independent determinations on different labeling experiments).