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. 1998 Nov 16;143(4):911–919. doi: 10.1083/jcb.143.4.911

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Analysis of topo II localization in asynchronous cells labeled with dUTP-F. dUTP-F was incorporated into endogenous gaps in kDNA by TdT, and cells were then processed for immunofluorescence. Topo II was visualized with goat anti–mouse antibody conjugated to Cy3 (Boehringer Mannheim Corp.). Images were captured as in Fig. 4. Top row, topo II immunofluorescence. Middle row, dUTP-F fluorescence of kDNA networks. Bottom row, DAPI fluorescence of kDNA networks. (a–d) Pre-replication networks. (e–g) Replicating kDNA networks. (h–k) Postreplication kDNA networks. (l–n) Cells containing two kDNA networks. Bar, 1 μm.