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. 1998 Nov 16;143(4):957–971. doi: 10.1083/jcb.143.4.957

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Ultrathin cryosections of CHO cells showing the subcellular localization of syntaxin 13. (A and B) Syntaxin 13 (10-nm gold) is present on extensive clusters of tubulovesicular membranes (arrowheads in A) located near Golgi (G) complex (A) or in association with early endosomes (EE). Sometimes continuities between EE vacuoles and syntaxin 13–positive tubules are seen (arrowheads in B). In addition, syntaxin 13 is found on EE vacuoles (B), often in parts of membrane that bear a dark cytosolic coating (arrows). (C) Double-immunogold labeling of syntaxin 13 (10-nm gold) and clathrin (15-nm gold) identifies the dark coating on the EE as clathrin. N, nucleus. Bars, 200 nm.