Figure 8.
Overexpression of titin Z1-Z2 or T-cap results in marked disruption of Z-discs. Cardiac myocytes transfected with a plasmid encoding the fusion proteins: (a and b) myc-titin Z1-Z2 and (c and d) myc–T-cap. Cells were fixed 48 h after transfection and double stained with (a and c) monoclonal anti-myc antibodies followed by FITC-conjugated donkey anti–mouse antibodies and (b and d) polyclonal anti–α-actinin antibodies followed by Texas red–conjugated F(ab′)2 fragments of donkey anti– rabbit antibodies. * marks cells that were not transfected. Bar, 10 μm.